DNA Vectors
System Biosciences LLC MSCV-hspCas9-EF1α-GFP Two Vector Cas9 SmartNuclease™ LentivectorCas9 expression vector for the Two Vector SmartNuclease System (MSCV-hspCas9-EF1α-GFP Lentivector Pre-Packaged Lentiviral Particles = >10^6 IFUs
When you want to create stable Cas9 editing cell lines and/or would like to edit the genome of a cell line that is resistant to transfection by plasmids, turn to one of SBI’s Cas9 SmartNuclease Lentivector Systems. Available as lentivector plasmids, ready-to-transduce pre-packaged pseudovirus, and in a package-your-own-lentivector kit with the lentivector plasmid and the LentiStarter 3.0 Packaging Kit, the MSCV-hspCas9-EF1α-GFP Two Vector Cas9 SmartNuclease™ Lentivector expresses human codon-optimized Cas9 wild-type nuclease from the strong MSCV promoter. The vector also includes a GFP marker expressed from the EF1α promoter to verify transduction efficiencies.
IBA LifeSciences pASK-IBA6C
pASK-IBA6C is a classic cloning vector devised for E. coli expression of recombinant proteins with the Strep-tagII fused to the N-terminus. It carries the chloramphenicol resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, a multiple cloning site with recognition sites for several common restriction enzymes (e.g. EcoRI, SmaI, BamHI, SalI, PstI), the inducible tetracycline promoter/operator for the regulated expression of proteins, the ompA signal for periplasmic secretion of the recombinant protein, and the sequence for a protease cleavage site.Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During the secretion into the periplasmic space the ompA signal sequence will be cleaved off. The N-terminal Strep-tagII can be removed by the protease factor Xa.
BIOHIPPO AAV-D2-fDIO-Cre-mCherry-WPRE-hGH-polyA
Recombinant adeno associated virus expressing Cre fused to mCherry under the D2 promoter with WPRE and hGH polyA elements. The Cre and Flp logic configuration enables expression only after recombination, providing precise targeted control of targeted neurons. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.
ABclonal Technology Rubicon Rabbit pAb
The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
BIOHIPPO AAV-U6-SpsgRNA(scramble)-CMV-EGFP-bGH-polyA
Recombinant adeno associated virus expressing SpsgRNA (scramble) fused to EGFP under the U6 CMV promoter with WPRE and hGH polyA elements. Offered under the Biohippo AAVX Series with multiple serotypes available including AAV2/1, AAV2/2, AAV2/5, AAV2/6, AAV2/6m, AAV2/6.2FF, AAV2/7m8, AAV2/8, AAV2/9, AAV2/DJ, AAV2/DJ8, AAV2/Retro, AAV2/PHP.eB, AAV2/PHP.B, AAV2/rh10, AAV2/PHP.S, AAV2/anc80, AAV2/B10, AAV2/olig001, AAV2/BI30, AAV2/BR1, AAV2/MG1.2, AAV1/2, AAV2/MaCPNS1, and AAV2/MaCPNS2. Regular titer is greater than or equal to 2.00E+12 vg per mL, with high titer greater than or equal to 1.00E+13 vg per mL available upon request at no additional cost. Customers may specify preferred serotype and titer when placing orders.